Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Ctcf

Cell type

Cell type Class
Blood
Cell type
G1E
Tissue
Blood
Lineage
cellLine
Description
Gata1- erythroid progenitor

Attributes by original data submitter

Sample

source_name
G1E cells
cell type
ES-derived erythroid progenitor cells
strain
C57BL/6
chip antibody
Millipore 07-729
growth protocol
GATA1-null proerythroblast cell line G1E (Weiss et al., 1997). G1E cells were grown in IMDM+15% FBS, kit ligand, monothioglycerol and Epoetin alpha in a standard tissue culture incubator at 37C degrees with 5% CO2 (as described in Weiss et al., 1997).

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's ChIP-seq Sample Preparation Kit.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
42587210
Reads aligned (%)
85.8
Duplicates removed (%)
27.6
Number of peaks
52439 (qval < 1E-05)

mm9

Number of total reads
42587210
Reads aligned (%)
85.6
Duplicates removed (%)
27.6
Number of peaks
52452 (qval < 1E-05)

Base call quality data from DBCLS SRA