GATA1-null proerythroblast cell line G1E (Weiss et al., 1997). G1E cells were grown in IMDM+15% FBS, kit ligand, monothioglycerol and Epoetin alpha in a standard tissue culture incubator at 37C degrees with 5% CO2 (as described in Weiss et al., 1997).
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were prepared from sonicated nuclei and protein-DNA complexes were isolated with antibody. ChIP-seq samples were prepared by end repair, 3' adenylation, and adaptor ligation using Illumina's ChIP-seq Sample Preparation Kit.